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goat anti platelet derived growth factor receptor beta  (R&D Systems)


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    R&D Systems goat anti platelet derived growth factor receptor beta
    Goat Anti Platelet Derived Growth Factor Receptor Beta, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+platelet+derived+growth+factor+receptor+beta/Mouse+PDGF+R+beta+Biotinylated+Antibody/pmc12869254-164-11-19
    Average 93 stars, based on 21 article reviews
    goat anti platelet derived growth factor receptor beta - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Cerebral cavernous malformations are driven by ADAMTS5 proteolysis of versican
    Article Snippet: .. The following primary antibodies were used for immunostaining: rabbit anti-versican (1:200, Millipore, AB1033), rabbit anti-DPEAAE (1:500, Pierce-Antibodies, PA1-1748A), rabbit anti-V5 (1:1,000, Abcam, AB9116), rat anti-platelet endothelial cell adhesion molecule (PECAM; 1:50; HistoBioTec, DIA-310), mouse anti-KLF4 (1:100, R&D, AF3158), rabbit anti-pMLC2 (1:200, Cell Signaling, 3674S), and goat anti-platelet-derived growth factor receptor beta (PDGFRB; 1:100, R&D, AF1042). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. METHOD DETAILS Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). .. Briefly, mice were anaesthetized with Avertin ( {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 , Sigma) and perfused through the right ventricle with phosphate-buffered saline (PBS, pH 7.4).

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Data-independent acquisition:

    Article Title: Cerebral cavernous malformations are driven by ADAMTS5 proteolysis of versican
    Article Snippet: .. The following primary antibodies were used for immunostaining: rabbit anti-versican (1:200, Millipore, AB1033), rabbit anti-DPEAAE (1:500, Pierce-Antibodies, PA1-1748A), rabbit anti-V5 (1:1,000, Abcam, AB9116), rat anti-platelet endothelial cell adhesion molecule (PECAM; 1:50; HistoBioTec, DIA-310), mouse anti-KLF4 (1:100, R&D, AF3158), rabbit anti-pMLC2 (1:200, Cell Signaling, 3674S), and goat anti-platelet-derived growth factor receptor beta (PDGFRB; 1:100, R&D, AF1042). ..

    Derivative Assay:

    Article Title: Chaperone-mediated autophagy sustains pericyte stemness necessary for brain tissue homeostasis
    Article Snippet: An automatic digital slide scanner (Pannoramic MIDI II-3DHistech) was used for acquisition of images. .. For fluorescent double labelling, mouse anti-alpha-smooth muscle actin (α-SMA; Abcam, ab7817), goat anti-platelet derived growth factor receptor beta (PDGFRβ; R&D Systems, BAF1042), or rat CD146-APC (Miltenyi Biotec, 130–118-408) antibodies were used in combination with rabbit anti-LAMP-2A (Invitrogen, 51–2200) antibody. .. Labelling was visualized by fluorescence microscopy using the corresponding secondary antibody conjugated to AlexaFluor488 (Invitrogen) or Cy5 (Invitrogen).

    Article Title: Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts
    Article Snippet: .. The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA, 1:1000, 14-1401-82, eBioscience), rat anti-cadherin-4 (CDH4, 1:20, MRCD5, Developmental Studies Hybridoma Bank), rat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, 14-1402-82, eBioscience), goat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, AF1042, R&D Systems), goat anti-notch3 (NOTCH3, 1:500, AF1308, R&D Systems), rabbit anti-mesenchyme homeobox 2 (MEOX2, 1:1000, NBP2-30647, Novus Biological), rabbit anti-cleaved caspase-3 (CASP3, 1:500, 9661, Cell Signaling), goat anti-peptidase inhibitor 16 (PI16, 1:1000, AF4929, R&D Systems), rat anti-protein tyrosine phosphatase, receptor type, C (CD45, 1:2000, 14-0451-81, eBioscience), Alexa488-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF488), Cy3-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Sigma, C6198), Alexa647-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF647), rat anti-advanced glycosylation end product-specific receptor (RAGE, 1:1000, R&D Systems, MAB1179), rabbit anti-transgelin (TAGLN, also known as SM22, 1:1000, Abcam, ab14106), chicken anti-green fluorescent protein (GFP, 1:5000, Abcam, AB13970), goat anti-hedgehog-interacting protein (HHIP, 1:1000, R&D Systems, AF1568), guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3, 1:500, Synaptic Systems, 391005), mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33, 1:500, R&D Systems, AF3626), rabbit anti-calponin (CNN1, 1:1000, Abcam, ab46794), and goat anti-delta/notch-like EGF repeat containing (DNER, 1:1000, AF2264, R&D Systems). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. METHOD DETAILS Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). .. Briefly, mice were anaesthetized with Avertin ( {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 , Sigma) and perfused through the right ventricle with phosphate-buffered saline (PBS, pH 7.4).

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Immunofluorescence:

    Article Title: Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts
    Article Snippet: .. The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA, 1:1000, 14-1401-82, eBioscience), rat anti-cadherin-4 (CDH4, 1:20, MRCD5, Developmental Studies Hybridoma Bank), rat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, 14-1402-82, eBioscience), goat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, AF1042, R&D Systems), goat anti-notch3 (NOTCH3, 1:500, AF1308, R&D Systems), rabbit anti-mesenchyme homeobox 2 (MEOX2, 1:1000, NBP2-30647, Novus Biological), rabbit anti-cleaved caspase-3 (CASP3, 1:500, 9661, Cell Signaling), goat anti-peptidase inhibitor 16 (PI16, 1:1000, AF4929, R&D Systems), rat anti-protein tyrosine phosphatase, receptor type, C (CD45, 1:2000, 14-0451-81, eBioscience), Alexa488-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF488), Cy3-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Sigma, C6198), Alexa647-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF647), rat anti-advanced glycosylation end product-specific receptor (RAGE, 1:1000, R&D Systems, MAB1179), rabbit anti-transgelin (TAGLN, also known as SM22, 1:1000, Abcam, ab14106), chicken anti-green fluorescent protein (GFP, 1:5000, Abcam, AB13970), goat anti-hedgehog-interacting protein (HHIP, 1:1000, R&D Systems, AF1568), guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3, 1:500, Synaptic Systems, 391005), mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33, 1:500, R&D Systems, AF3626), rabbit anti-calponin (CNN1, 1:1000, Abcam, ab46794), and goat anti-delta/notch-like EGF repeat containing (DNER, 1:1000, AF2264, R&D Systems). ..

    Glycoproteomics:

    Article Title: Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts
    Article Snippet: .. The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA, 1:1000, 14-1401-82, eBioscience), rat anti-cadherin-4 (CDH4, 1:20, MRCD5, Developmental Studies Hybridoma Bank), rat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, 14-1402-82, eBioscience), goat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, AF1042, R&D Systems), goat anti-notch3 (NOTCH3, 1:500, AF1308, R&D Systems), rabbit anti-mesenchyme homeobox 2 (MEOX2, 1:1000, NBP2-30647, Novus Biological), rabbit anti-cleaved caspase-3 (CASP3, 1:500, 9661, Cell Signaling), goat anti-peptidase inhibitor 16 (PI16, 1:1000, AF4929, R&D Systems), rat anti-protein tyrosine phosphatase, receptor type, C (CD45, 1:2000, 14-0451-81, eBioscience), Alexa488-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF488), Cy3-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Sigma, C6198), Alexa647-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF647), rat anti-advanced glycosylation end product-specific receptor (RAGE, 1:1000, R&D Systems, MAB1179), rabbit anti-transgelin (TAGLN, also known as SM22, 1:1000, Abcam, ab14106), chicken anti-green fluorescent protein (GFP, 1:5000, Abcam, AB13970), goat anti-hedgehog-interacting protein (HHIP, 1:1000, R&D Systems, AF1568), guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3, 1:500, Synaptic Systems, 391005), mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33, 1:500, R&D Systems, AF3626), rabbit anti-calponin (CNN1, 1:1000, Abcam, ab46794), and goat anti-delta/notch-like EGF repeat containing (DNER, 1:1000, AF2264, R&D Systems). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. METHOD DETAILS Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). .. Briefly, mice were anaesthetized with Avertin ( {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 , Sigma) and perfused through the right ventricle with phosphate-buffered saline (PBS, pH 7.4).

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Membrane:

    Article Title: Three-axis classification of mouse lung mesenchymal cells reveals two populations of myofibroblasts
    Article Snippet: .. The following antibodies were used for immunofluorescence: goat anti-platelet derived growth factor-alpha (PDGFRA, 1:1000, AF1062, R&D Systems), rat anti-PDGFRA (PDGFRA, 1:1000, 14-1401-82, eBioscience), rat anti-cadherin-4 (CDH4, 1:20, MRCD5, Developmental Studies Hybridoma Bank), rat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, 14-1402-82, eBioscience), goat anti-platelet derived growth factor receptor-beta (PDGFRB, 1:1000, AF1042, R&D Systems), goat anti-notch3 (NOTCH3, 1:500, AF1308, R&D Systems), rabbit anti-mesenchyme homeobox 2 (MEOX2, 1:1000, NBP2-30647, Novus Biological), rabbit anti-cleaved caspase-3 (CASP3, 1:500, 9661, Cell Signaling), goat anti-peptidase inhibitor 16 (PI16, 1:1000, AF4929, R&D Systems), rat anti-protein tyrosine phosphatase, receptor type, C (CD45, 1:2000, 14-0451-81, eBioscience), Alexa488-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF488), Cy3-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Sigma, C6198), Alexa647-conjugated mouse anti-smooth muscle actin (ACTA2, 1:1000, Santa Cruz Biotechnology, sc-32251 AF647), rat anti-advanced glycosylation end product-specific receptor (RAGE, 1:1000, R&D Systems, MAB1179), rabbit anti-transgelin (TAGLN, also known as SM22, 1:1000, Abcam, ab14106), chicken anti-green fluorescent protein (GFP, 1:5000, Abcam, AB13970), goat anti-hedgehog-interacting protein (HHIP, 1:1000, R&D Systems, AF1568), guinea pig anti-lysosomal-associated membrane protein 3 (LAMP3, 1:500, Synaptic Systems, 391005), mouse anti-NK2 homeobox 1 (NKX2-1, 1:500, Leica Biosystems, TTF-1-L-CE), goat anti-interleukin 33 (IL33, 1:500, R&D Systems, AF3626), rabbit anti-calponin (CNN1, 1:1000, Abcam, ab46794), and goat anti-delta/notch-like EGF repeat containing (DNER, 1:1000, AF2264, R&D Systems). ..

    Virus:

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. METHOD DETAILS Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). .. Briefly, mice were anaesthetized with Avertin ( {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 , Sigma) and perfused through the right ventricle with phosphate-buffered saline (PBS, pH 7.4).

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Western Blot:

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. METHOD DETAILS Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). .. Briefly, mice were anaesthetized with Avertin ( {"type":"entrez-nucleotide","attrs":{"text":"T48402","term_id":"650382","term_text":"T48402"}} T48402 , Sigma) and perfused through the right ventricle with phosphate-buffered saline (PBS, pH 7.4).

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..

    Article Title: Epithelial Vegfa specifies a distinct endothelial population in the mouse lung
    Article Snippet: .. Antibodies The following antibodies were used: rabbit anti-Aquaporin 5 (AQP5, 1:2500, ab78486, Abcam), goat anti-Carbonic anhydrase IV (CAR4, 1:500, AF2414, R&D), rat anti-mouse CD31 (1mg/mL, 550274, BD Biosciences), BV786 rat anti-CD31 (1:250, 740870, BD Biosciences), PE/Cy7 rat anti-CD45 (1:250, 103114, BioLegend), mouse anti-Claudin 5 (Cldn5, 1:500, 352588, Invitrogen), rabbit anti-collagen IV (COL4, 1:2500, LSL-LB-1403, CosmoBioUSA), goat anti-Delta like canonical Notch ligand 4 (DLL4, 1:250, AF1389, R&D), Alexa Fluor 488 rat anti-CD324 (ECAD, 1:500, 53-3249-80, eBioscience), goat anti-Elastin (ELN, 1:500, a gift from Dr. Barry Starcher), goat anti-Endothelial cell specific molecule 1 (ESM1, 1:500, AF1999, R&D), rat anti-endomucin (EMCN, 1:2500, 14-5851-81, eBioscience), rabbit anti-Avian erythroblastosis virus E-26 (v-ets) oncogene related (ERG, 1:5000 for both immunostaining and western blot, ab92513, Abcam), goat anti-Vegfr3/Flt4 (1:1000, AF743, R&D), chicken anti-beta Galactosidase (LacZ, 1:500, Ab9361, Abcam), chicken anti-Green fluorescent protein (GFP, 1:5000, AB13970, Abcam), rabbit anti-Histone H3 (H3, 1:2000 for western blot, 4499, Cell Signaling), Alexa Fluor 647 rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:500, A15452, ThermoFisher), rat anti-Intercellular adhesion molecule 2 (ICAM2, 1:2500, 16-1021-82, eBioscience), goat anti- Intercellular adhesion molecule 2 (ICAM2, 1:500, AF774, R&D systems), eFluor 570 rat anti-Ki67 (1:500, 41-5698-82, eBioscience), rabbit anti-Ki67 (1:1000, RM9106S0, ThermoFisher), rabbit anti-Chondroitin sulfate proteoglycan 4 (CSPG4, 1:1000, AB5320, Millipore), rabbit anti-NK2 Homeobox 1 (NKX2.1, 1:1000, sc-13040, Santa Cruz), rat anti-Platelet derived growth factor receptor alpha (PDGFRA, 1:1000, 14-1401-82, eBioscience), goat anti-Platelet derived growth factor receptor beta (PDGFRB, 1:1000, AF1042, R&D systems), rat anti-Plasmalemma vesicle associated protein (PLVAP, 1:125, 553849, BD Biosciences), rabbit anti-Prospero Homeobox 1 (PROX1, 1:250, 11–002, AngioBio), rat anti-Advanced glycosylation end-product specific receptor (RAGE, 1:1000, MAB1179, R&D systems), rabbit anti-Red fluorescent protein (RFP, 1:1000, 600-401-379, Rockland), Cy3-conjugated mouse anti-alpha-Smooth muscle actin (SMA, 1:1000, C6198, Sigma), rabbit anti-SM22 (TAGLN, 1:2500, ab14106, Abcam), Alexa Fluor 647 rat anti-Vascular endothelial cadherin (VECAD/CDH5, 1:250, 562242, BD Biosciences), rabbit anti-phospho-VEGF Receptor 2 (Tyr1175) (p-VEGFR2, 1:1000 for western blot, 2478, Cell Signaling), rabbit anti-VEGF Receptor 2 (VEGFR2, 1:5000 for western blot, 9698, Cell Signaling), rabbit anti-Von Willebrand Factor (VWF, 1:2500, ab6994, Abcam). ..



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